Alterations in gastric juice free amino acid spectrum in experimental chronic necrotic pancreatitis

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O. A. Grinchenko
S. P. Veselsky
Z. A. Gorenko

Abstract

Chronic pancreatitis is characterized by destruction of pancreatic secretory parenchyma, progressive exocrine and endocrine insufficiency, as well as a various complications including gastric dysfunctions. At the pathology can changes the ratio of amino acids in bioliquids. Therefore the aim of the work was to investigate the gastric juice free amino acid spectrum features in experimental chronic necrotic pancreatitis. Chronic pancreatitis in rats modeled using L-arginine. Gastric content obtained by aspiration method at 10 and 63 days after pancreatitis induction, free amino acid levels were determined using the method of paper chromatography and densitometer DO-1М. It was established that the expression of amino acids content changes depends on the pathology course duration. On the 10 day the individual levels of ornithine, lysine, arginine, glutamic acid, threonine, proline, oxyproline, valine and tyrosine are increased. As at 10 and 63 days the concentration of sulfur-containing amino acids, histidine and histamine, aspartic acid and asparagine, glycine, alanine, leucine and isoleucine, phenylalanine and tryptophan are significantly reduced, which progresses with the course of pathology. As a result, 2 months after the induction of pancreatitis the total pool of gastric juice free amino acid is reduced. These changes are accompanied by distinct amino acid imbalance. Redistribution in the gastric content free amino acids spectrum is likely to affect the exocrine function of the stomach.

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References

Грінченко О.А. Роль оксиду азоту і таурину у регуляції секреторної функції шлунка собак (The role of nitric oxide and taurine in regulation of dogs gastric secretory function) / О.А. Грінченко, П.І. Янчук // Фізіологічний журнал. - 2012. – Т. 58, № 6. - С. 48-56.

Губергриц Н.Б. Место фамотидина в лечении хронического панкреатита (Famotidine place in the treatment of chronic pancreatitis) / Губергриц Н.Б., Слесарева К.Н. // Сучасна гастроентерологія. – 2009. - № 2(46). – С. 73-80.

Коробейникова Э.М. Определение содержания свободных аминокислот в сыворотке крови и моче здоровых детей (Determination of free amino acids in the serum and urine of healthy children) / Коробейникова Э.М., Мeщeрякова Г.В. // Лаб.дело. – 1981. - № 4. – С. 221-224.

Кочетов Г.А. Практическое руководство по энзимологии (A practical guide to enzymology) / Кочетов Г.А. ; под ред. С.Е. Северина. - М. : Высшая школа, 1980. – 271 с.

Маев И.В. Желудочное кислотообразование и хронический панкреатит: насколько сильна взаимосвязь? (Gastric acid production and chronic pancreatitis: how strong is the mutual relationship?) / Маев И.В., Кучерявый Ю.А. // РЖГГК. – 2008. - № 3. С. 4-14.

Степанов Ю.М. Особливості секреторної функції та характер мікробної контамінації вмісту шлунка за різних форм хронічного панкреатиту (The peculiarities of secretory function and character of microbial contamination of the stomach in various forms of chronic pancreatitis) / Степанов Ю.М., Крилова О.О., Руденко А.І. [та ін.] // Сучасна гастроентерологія. – 2013. - № 3 (71). – С. 33-39.

Aghdassi A.A. Animal model for investigating chronic pancreatitis / Aghdassi A.A., Mayerle J., Christochowitz S. [et al.] // Fibrogenesis & tissue repair. – 2011. - Vol. 4, № 1. – Р. 26.

Alcaraz-Conteras Y. Effect of glycine on lead mobilization, lead-induced oxidative stress, and hepatic toxicity in rats / Alcaraz-Conteras Y., Garza-Ocañas L., Carcaño-Diaz K., Ramirez-Gomez X.S. // J. Toxicol. – 2011. – Vol. 201. – ID430539. – 7 p.

Berg A. Nitric oxide inhibits gastric acid secretion by increasing intraparietal cell levels of cGMP in isolated human gastric glands / Berg A., Redeen S., Grenegard M. [et al.] // AJP: Gastrointest. Liver Physiol. – 2005. – Vol. 289. – P. G1061-G1066.

Blum T. Fatal outcome in acute pancreatitis: its occurrence and early prediction / Blum T., Maisonneuve P., Lowenfels A.B., Lankish P.G. // Pancreatology. – 2001. - Vol. 1, № 3. – Р. 237-241.

Browne G.W. Pathophysiology of pulmonary complications of acute pancreatitis / Browne G.W., Pitchumoni C.S. // World J. Gastroenterol. – 2006. - Vol. 12, № 44. – Р. 7078-7096.

Campbell C.A. Validation of a conscious rat model for the discovery of novel agents that inhibit gastric acid secretion / C.A. Campbell, P.J. Gaskin, J. Darton [et al.] // Eur. J. Pharmacol. – 2008. – Vol. 589, № 1-3. – P. 260-263.

Cao M. Cannabinoid HU210 protect isolated rat stomach against impairment caused by serum of rats with experimental acute pancreatitis / Cao M., Li J., Xu J. [et al.] // PLoS ONE. – 2012. - Vol. 7, № 12. – Р. e52921.

Delaney C.P. Pancreatic atrophy: a new model using serial intra-peritoneal injections of L-arginine / Delaney C.P., McGeeney K.F., Dervan P., Fitzpatrick J.M. // Scand. J. Gastroenterol. – 1993. - Vol. 28, № 12. – Р. 1086-1090.

Deng K. High levels of aromatic amino acids in gastric juice during the early stages of gastric cancer progression / Deng K., Lin S., Zhou L. [et al.] // PLoS One. – 2012. – Vol. 7, № 11. – P. e49434. (doi: 10.1371/journal.pone.0049434.)

Deng K. Three aromatic amino acids in gastric juice as potential biomarkers for gastric malignancies / Deng K., Lin S., Zhou L. [et al.] // Anal. Chim. Acta. – 2011. – Vol. 694, № 1-2. - P. 100-107.

Folch-Puy E. Importance of the liver in systemic complications associated with acute pancreatitis: the role of Kupffer cells / Folch-Puy E. // J. Pathol. – 2007. - Vol. 211, № 4. – Р. 383-388.

Girish B.N. Аlterations in plasma amino acid levels in chronic pancreatitis / B.N. Girish, G. Rajesh, K. Vaidyanathan [et al.] // JOP. J. Pancreas. – 2011. – Vol. 12, № 1. – P. 11-18.

Goo T. Mechanisms of intragastric pH sensing / Goo T., Akiba Y., Kaunitz J.D. // Curr. Gastroenterol. Rep. – 2010. – Vol. 12, № 6. – P. 465-470.

Hegyi P. L-arginine-induced experimental pancreatitis / Hegyi P., Raconzay Z., Sari R. [et al.] // World J. Gastroenterol. – 2004. - Vol. 10, № 14. – Р. 2003-2009.

Hegyi P. Spontaneous and cholecystokinin-octapeptide-promoted regeneration of the pancreas following L-arginine-induced pancreatitis in rat / Hegyi P., Takacs T., Jarmay K. [et al.] // Int. J. Pancreatol. – 1997. - Vol. 22, № 3. – Р. 193-200.

Khropycheva R. Dietary glutamate signal evokes gastric juice excretion in dogs / Khropycheva R., Andreeva J., Uneyama H. [et al.] // Digestion. – 2011. – Vol. 83, Suppl. 1. – P. 7-12.

Kirchhoff P. An amino acid transporter involved in gastric acid secretion / P. Kirchhoff, M.H. Dave, C. Remy // Pflügers Archiv. – 2006. – Vol. 451, Is. 6. – P. 738-748.

Lechin F. Arginine-induced pancreatitis: involvement of the autonomic nervous system? / F. Lechin, B. Dijs // AJP: Gastrointest. Liver Physiol. – 2008. - Vol. 294. – P. G1450-1451.

Lee M.A. Influence of glycine on intestinal ischemia-reperfusion injury / Lee M.A., McCauley R.D., Kong S.E., Hall J.C. // J.Parenter. Enteral. Nutr. – 2002. – Vol. 26. – P. 130-135.

Mikalauskas S. Dietary glycine protects from chemotherapy-induced hepatotoxicity / Mikalauskas S., Mikalauskiene L., Bruns H. [et al.] // Amіno Acids. – 2011. – Vol. 40, Is. 3. – P. 1139-1150.

Nagata Y. High concentrations of D-amino acids in human gastric juice / Y. Nagata, T. Sato, N. Enomoto [et al.] // Amino Acids. – 2007. – Vol. 32, Is. 1. – P. 137-140.

Nakamura E. Luminal amino acid-sensing cells in gastric mucosa / Nakamura E., Hasumura M., Uneyama H. [et al.] // Digestion. – 2011. – Vol. 83, Suppl. 1. – P. 13-18.

Otsuki M. Animal models of chronic pancreatitis / Otsuki M., Yamamoto M, Yamaguchi T. // Gastroenterol. Res. Pract. – 2010. - Article ID 403295, 8 p. Doi:10.1155/2010/403295.

Tani S. New model of acute necrotizing pancreatitis induced by excessive doses of arginine in rats / Tani S., Itoh H., Okabayaschi Y. [et al.] // Dig. Dis. Scien. – 1990. - Vol. 35, № 3. – Р. 367-374.

Tsune I. Dietary glycine prevents chemical-induced experimental colitis in the rat / Tsune I., Ikejima K., Hirose M. [et al.] // Gastroenterology. – 2003. – Vol. 125, Is. 3. – P. 775-785.

Wang W.W. Amino acids and gut function / Wang W.W., Qiao S.Y., Li D.F. // Amino acids. – 2009. – Vol. 37. – P. 105-110.

Wang Y. Amino acids stimulate cholecystokinin release through the Ca2+-sensing receptor / Wang Y., Chandra R., Samsa L.A. [et al.] // AJP: Gastrointest. Liver Physiol. – 2011. – Vol. 300. – P. G528-G537.

Wu G. Glutathione metabolism and its implication for health / Wu G., Fang Y.Z., Yang S. [et al.] // J. Nutr. – 2004. – Vol. 1345. – P. 489-492.

Yasuda K. Insulin responses to administrations of amino acids and fatty acids in healthy cats / Yasuda K., Takashima S., Takagi M. [et al.] // J. Vet. Med. Scien. – 2011. – Vol. 73, № 10. – Р. 1281-1286.

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