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Introduction. Bile acids are specific and important physiologically active metabolic products of cholesterol transformation in hepatocytes. Cholates concentrations in bile reflect the intensity of the biotransformation, synthesis and transport of bile components in the liver tissue. The conversion of cholesterol to bile acids necessary to maintain the sustainability of cholesterol in the blood and prevents the accumulation of cholesterol, triglycerides, toxic metabolites in the liver cells and other organs. So it is important to study effects of various drugs on the metabolism of cholesterol under experimental hypercholesterolemia. Drugs that can normalize the metabolism of cholesterol in the liver and bile acid synthesis from cholesterol in hepatocytes include flavonoids such as quercetin.
Purpose. The aim of our work was to study the Corvitin (water-soluble form of quercetin) action content of bile acids in the bile of rats with experimental doxycycline hypercholesterolemia.
Methods. The studies used animals of three groups: the first – control (intragastric introduction of water during the whole time of the experiment), the second – with experimental hypercholesterolemia (intragastric administration of doxycycline at a dose of 540 mg/kg body weight), the third group – animals with experimental hypercholesterolemia and weekly oral Corvitin at a dose of 1 mg/kg body weight. Bile secretion liver function was studied in acute experiments for all animals groups. Sodium thiopental at a dose of 70 mg/kg was used for anesthesia of animals. In acute experiments, anesthetized rats laparotomy was performed and bile duct cannulated. Then for three hours six samples of bile were collected. Separate fractions of conjugated bile acids (taurocholate, taurochenodeoxycholate and taurodeoxycholate, glycocholate, glycochenodeoxycholate and glycodeoxycholate) and free bile acids (cholate, chenodeoxycholate and deoxycholate) were determined by TLC in samples of bile.
Results. It was found that the taurocholate concentration (content of this bile acid is highest in the bile of rats) decreased at doxycycline hypercholesterolemia and the after Corvitin application. Glycocholate concentration decreased under the doxycycline influence. But after the Corvitin application glycocholate concentration in bile increased and reached baseline. Also Corvitin normalized taurohydroxycholates and glycohydroxycholates bile concentration in rats with doxycycline hypercholesterolemia. There were also identified changes in the content of free (non-conjugated) bile acids in the bile of rats. Cholic acid concentration decreased under the Corvitin and doxycycline influences. But Corvitin significantly increased bile concentration of the free dyhydroxycholates (chenodeoxycholic acids and deoxycholic acids) in doxycycline hypercholesterolemia rats.
Originality. It was firstly shoud that (Corvitin) normalized content of the conjugated bile acids (except taurocholic bile acids) in the male rats bile at doxycycline hypercholesterolemia. Also Corvitin caused decreasing of the cholic acid concentrration and increasing of the chenodeoxycholic and deoxycholic acids concentration in the bile of the rats at doxycycline hypercholesterolemia.
Conclusion. Taurocholic acid concentration decreased both under hypercholesterolemia modeling with the help of the doxycycline and using Corvitin. Corvitin normalized concentrationof the glycocholic acids in rats at doxycycline hypercholesterolemia. Usage of the Corvitin for rats at doxycycline hypercholesterolemia promoted normalization of the tauro- and glyco- dihydroxycholic bile acids concentration. Corvitin caused decreasing of the cholic acid concentration, but it leaded to significant increasing of the free dihydroxycholic bile acids concentration in bile of rats at doxycycline hypercholesterolemia. So, сorvitin can significantly influence the bile acids composition of bile in rats with experimental hypercholesterolemia.
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Chiang, J. Y. (2013). Bile acid metabolism and signaling. (Compr Physiol).
Dawson, P.A., Karpen, S.J. (2015). Intestinal transport and metabolism of bile acids. (J. Lipid Res).
Li, T., Chiang, J.Y. (2014). Bile acid signaling in metabolic disease and drug therapy. (Pharmacol Rev).
Li, T., Chiang, J.Y. (2015). Bile acids as metabolic regulators. (Curr Opin Gastroenterol).
Ma, H., Patti, M.E. (2014). Bile acids, obesity, and the metabolic syndrome. (Best Pract Res Clin Gastroenterol).
Bueverov, A.O. (2001). Medicinal liver damage. Rossiyskiy meditsinskiy zhurnal. (Russian Medical Journal), 9, 13-14. (in Russ.)
Tang, D.M., Koh, C., Twaddell, W.S., Rosenvinge, E.C., Han, H. (2015). Acute Hepatocellular Drug-Induced Liver Injury From Bupropion and Doxycycline. (ACG Case Rep J.)
Amanova, G.N., Orel, N.M., Chubarov, S.I. (2008). Effect of low-intensity infrared laser radiation with a wavelength of 847 nm on LDH activity in blood serum and liver of rats with doxycycline-induced cholestasis. Ksenobiotiki i zhivyie sistemyi: materialyi III Mezhdunar. nauch. konf. Minsk: Izd. tsentr BGU. (in Russ.)
Kholmukhamedov, A., Czerny, C., Hu, J. (2014). Minocycline and doxycycline, but not tetracycline, mitigate liver and kidney injury after hemorrhagic shock/resuscitation. (Shock)
Oryol, N.M., Chubarov, S.I., Avtomeenko, O.L. (2007). Effect of radiation of semiconductor lasers with wavelengths 877 nm, 847 nm and 670 nm on cholesterol level in blood serum and tissues of rats with experimental hypercholesterolaemia. Signalnyie mehanizmyi regulyatsii fiziologicheskih funktsiy: sb.nauch.st. Minsk: RIVSh. (in Russ.)
Gordienko, A.D. (1990). Mechanism of hepatoprotective flavonoids action. Farmatsiya (Pharmacy), 3, 75-79. (in Russ.)
Vovkun, T.V., Yanchuk, P.I., Shtanova, L.Ia., Veselskyi, S.P., Shalamai, A.S. (2016). Exocrine function of the liver in rats with exposure to Cоrvitin. Fiziolohichnyi zhurnal (Physiological journal), 3, 30-38. (in Ukr.)
Veselskiy, S.P., Lyaschenko, P.S., Lukyanenko, I.A.. A method of determining bile acids in biological fluids: A.s.4411066/14 SSSR, MBI G 01 N 33/50. #1624322; Zayavleno 25.01.1988; Opubl. 30.01.1991, Byul. # 4. (in USSR)
Corso, G., Papagni, F., Gelzo, M. et al (2015). Development and Validation of an Enzymatic Method for Total Cholesterol Analysis Using Whole Blood Spot. (J. Clin. Lab. Anal)
Filimonova, N.B., Fil, I.O., Mykhailova, T.S. (2004). Statistical analysis of data pursuant to the science-based medicine. Initial analysis of quantitative data, experimental results presentation. Pervynnyi analiz kilkisnykh danykh, podannia rezultativ eksperymentu. Medytsyna zaliznychnoho transportu Ukrainy (Medicine Railway Transport of Ukraine), 4, 30-38. (in Ukr.)
Filimonova, N.B., Fil, I.O. (2005). Statistical analysis of data pursuant to the science-based medicine. Comparison groups in quantitative terms. Medytsyna transportu Ukrainy (Medical transport Ukraine), 4, 86-93. (in Ukr.)