Influence of medicine “Cordyceps & Lingzhi” and its fractions on some indexes of immune system, tumor growth and survival of mice with Ehrlich’s carcinoma
Main Article Content
Abstract
Fungotherapy is a well-developed method of therapy in East Asia. Nowadays there is the search and study of the main active substances with different pharmacological activities from higher fungi takes place. The aim of our study was to compare the immunomodulatory and antitumor activity of the medicine "Cordyceps and Lingzhi" and water-and fat-soluble fractions derived from it. In vitro studies conducted using cells obtained from outbred mice. In vivo studies conducted on Balb/c mice. Functional activity of phagocytes was determined using the nitroblue tetrazolium test. The reaction of lymphoid organs was measured by indexes of their relative weight and relative cellularity. The presence of immunomodulating activity of “Cordyceps & Lingzhi” medicine water and fat soluble fractions was shown. Changes of oxygen-dependent metabolism were the most reliable, they were increased at addition of analyzed fractions in all concentrations. Nevertheless, application of non-fractionated medicine “Cordyceps & Lingzhi” had more marked effects in almost all investigated parameters, such as oxygen-dependent metabolism (19-fold increase at addition of 100 mkg/ml of medicine, as compared to control), indexes of lymphoid organs (1,5-fold increase of relative weight of lymph nodes), dynamics of tumor growth (statistically significant inhibition of tumor growth by 24% as compared to control).
Article Details
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
AGREEMENT ABOUT TRANSMISSION OF COPYRIGHT
I, the author of the article / We, the authors of the manuscript _______________________________________________________________________
in case of its acceptance for publication, we transfer the following rights to the founders and editorial boards of the scientific publication "Cherkasy University Bulletin: Biological Sciences Series":
1. Publication of this article in Ukrainian (English) and distribution of its printed version.
2. Dissemination of the electronic version of the article through any electronic means (placing on the official journal web site, in electronic databases, repositories, etc.). At the same time we reserve the right without consent of the editorial board and the founders:
1. Use the materials of the article in whole or in part for educational purposes.
2. To use the materials of the article in whole or in part for writing your own theses.
3. Use article materials to prepare Summarys, conference reports, and oral presentations.
4. Post electronic copies of the article (including the final electronic version downloaded from the journal's official website) to:
a. personal web-pecypcax of all authors (web sites, web pages, blogs, etc.);
b. web-pecypcax of the institutions where the authors work (including electronic institutional repositories);
with. non-profit, open-source web-pecypcax (such as arXiv.org).
With this agreement, we also certify that the submitted manuscript meets the following criteria:
1. Does not contain calls for violence, incitement of racial or ethnic enmity, which are disturbing, threatening, shameful, libelous, cruel, indecent, vulgar, etc.
2. Does not infringe the copyrights and intellectual property rights of others or organizations; contains all the references to the cited authors and / or publications envisaged by applicable copyright law, as well as the results and facts used in the article by other authors or organizations.
3. It has not been previously published in other publishers and has not been published in other publications.
4. Does not include materials that are not subject to publication in the open press, in accordance with applicable law.
____________________ ___________________
First name, Last name, signature of the author
"___" __________ 20__
References
Patel S. Recent developments in mushrooms as anti-cancer therapeutics: a review / S. Patel, A. Goyal // Biotech. – 2012. – V. 2, №1. – P. 1–15.
Zhou X. Cordyceps fungi: natural products, pharmacological functions and developmental products / X. Zhou, Z. Gong, Y. Su [et al.] // Journal of Pharmacy and Pharmacology. – 2009. – V. 61. – P. 279 – 291.
Rao Y.-K. Constituents isolated from Cordyceps militaris suppress enhanced inflammatory mediator’s production and human cancer cell proliferation / Y.-K. Rao, S.-H. Fang, W.-S. Wu, Y.-M. Tzeng – .J Ethnopharmacol. – 2010. – V. 131. – P. 363–367.
Chen J. Medicinal importance of fungal β-(1 → 3) (1 → 6)-glucans / J. Chen, R. Seviour // Mycol. Res. – 2007. – V. 111. – P. 635–652
Wang P-Y. Antitumor and Immunomodulatory Effects of Polysaccharides from Broken-Spore of Ganoderma lucidum / P.-Y. Wang, X.-L. Zhu, Z.-B. Lin // Front Pharmacol. – 2012. – V. 3. – P. 135.
Lin S.B. Triterpene-enriched extracts from Ganoderma lucidum inhibit growth of hepatoma cells via suppressing protein kinase C, activating mitogen-activated protein kinases and G2-phase cell cycle arrest / S.B. Lin, C.H. Li, S.S. Lee, L.S. Kan // Life Sci. – 2003. – V. 72. – P. 2381 – 2390.
Галиев Х.Г. Микрометод определения ароматических аминокислот в субклеточных структурах печени / Х.Г. Галиев // Экспериментальная патология печени. – 1981. – №4. – С. 245 – 250.
Пат. 99031324 Україна, А 61В 5/14 Спосіб підготовки проб біорідин для визначення вмісту речовин ліпідної природи / С.П. Весельський; Київський Університет імені Тараса Шевченка. 15.02.2001. Бюл. №1.
Резников О. Проблеми етики при проведенні експериментальних медичних і біологічних досліджень на тваринах / О. Резников // Вісн. НАН України. – 2001. - №1. – С. 5-7.
Sensitivity of mouse lymphoid and nonlymphoid organs to Silesian air pollutants / E. Kozlowska, J. Kopec-Szlezak, N. Drela// Ecotoxicol. Environ. Saf. - 1997. - №37. - №1. - P.10-16.
Реброва О.Ю. Статистический анализ медицинских данных / О.Ю. Реброва. – Москва: МедиаСфера, 2002. – 312 с.