Alterations in gastric juice free amino acid spectrum in experimental chronic necrotic pancreatitis

O. A. Grinchenko, S. P. Veselsky, Z. A. Gorenko

Abstract


Chronic pancreatitis is characterized by destruction of pancreatic secretory parenchyma, progressive exocrine and endocrine insufficiency, as well as a various complications including gastric dysfunctions. At the pathology can changes the ratio of amino acids in bioliquids. Therefore the aim of the work was to investigate the gastric juice free amino acid spectrum features in experimental chronic necrotic pancreatitis. Chronic pancreatitis in rats modeled using L-arginine. Gastric content obtained by aspiration method at 10 and 63 days after pancreatitis induction, free amino acid levels were determined using the method of paper chromatography and densitometer DO-1М. It was established that the expression of amino acids content changes depends on the pathology course duration. On the 10 day the individual levels of ornithine, lysine, arginine, glutamic acid, threonine, proline, oxyproline, valine and tyrosine are increased. As at 10 and 63 days the concentration of sulfur-containing amino acids, histidine and histamine, aspartic acid and asparagine, glycine, alanine, leucine and isoleucine, phenylalanine and tryptophan are significantly reduced, which progresses with the course of pathology. As a result, 2 months after the induction of pancreatitis the total pool of gastric juice free amino acid is reduced. These changes are accompanied by distinct amino acid imbalance. Redistribution in the gastric content free amino acids spectrum is likely to affect the exocrine function of the stomach.

Keywords


chronic pancreatitis; gastric juice; free amino acids

References


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