uk Evaluation of the Effectiveness of ISSR-Marker Informativeness Indicators for the Analysis of Plant Genetic Polymorphism
Main Article Content
Abstract
A number of generations of different types of molecular genetic markers have changed to
the present day.
The DNA nucleotide sequence marker system allows testing genetic diversity at the gene level. The
creation of molecular markers and their use in biological researches made it possible to study genetic
polymorphism in more detail, as well as to investigate the level of kinship at the inter- and intra-species levels.
The use of various DNA markers that are associated with different sequences of the genome is effective at the
time of studying genetic polymorphism. At the same time, it is also important to reduce the cost of polymerase
chain reaction analysis, which is associated with the use of a small number of primers, and to choose the most
informative primers.
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References
Сиволап Ю.М. Геном рослин і «молекулярна селекція». Селекція і насінництво. 2008. Т. 96. С. 34–42.
Misra A., Shasany A.K., Shukla A.K. et al. AFLP markers for identification Swertia species (Gentianaceae).
Genetics and Molecular Research. 2010. Vol. 9 (3). P. 1535–1544.
Mondini L., Noorani A., Pagnotta M.A. Assessing plant genetic diversity by molecular tools. Diversity. 2009.
Vol. 1. P. 19–35.
Чесноков Ю.В., Артемьева А.М. Оценка меры информационного полиморфизма генетического
разнообразия. Сельскохозяйственная биология. 2015. Т. 50, № 5. С. 571–578.
Amiryousefi A., Hyvönen J., Poczai P. iMEC: Online Marker Efficiency Calculator. Applications in Plant
Sciences. 2018. Vol. 6 (6). e1159.
Powell W., Morgante M., Andre C., Hanfey M., Vogel J., Tingey S., Rafalski A. The comparison of RFLP,
RAPD, AFLP and SSR (microsatellite) markers for germplasm analysis. Mol. Breeding. 1996. 2. P. 225–228.
Prevost A., Wilkinson M.J. A new system of comparing PCR primers applied to ISSR fingerprinting of potato
cultivars. Theor. Appl. Genet. 1999. 98. P. 107–112.
Tessier C., David J., This P., Boursiquot J.M., Charrier A. Optimization of the choice of molecular markers for
varietal identification in Vitis vinifera L. Theor. Appl. Genet. 1999. 98. P. 171–177.
Rogers S.O., Bendich A.J. Extraction of DNA from milligram amounts of fresh herbarium and mummified plant
tissues. Plant Mol. Biol. 1985. Vol. 5. P. 69–76.
Спірідонова К.В. Вивчення особливостей геномної мінливості культивованих клітин раувольфії зміїної
Rauwolfia serpentina Benth : дис...канд. біол. наук: 03.00.15. Київ, 2000. 149 с.
Mosula M.Z., Konvalyuk I.I., Mel’nyk V.M., Drobyk N.M., Tsaryk Y.V., Nesteruk Yu.Y., Kunakh V.A. Genetic
polymorphism of Gentiana lutea L. (Gentianaceae) populations from the Chornohora ridge of the Ukrainian
Carpathians. Cytology and Genetics. 2014. Vol. 48, № 6. P. 371–377. Doi: 10.3103/S0095452714060073.
Андрєєв І.О., Мосула М.З., Мельник В.М., Бублик О.М., Конвалюк І.І., Дробик Н.М. Порівняння
показників інформативності ПЛР-маркерів для аналізу генетичного різноманіття на прикладі Gentiana
lutea L. Фактори експериментальної еволюції організмів. 2014. Т. 14. С. 141–146.
Saini M., Singh S., Hussain Z. et al. RAPD analysis in mungbean [Vigna radiata (L.) Wilczek.] II: A comparison
of efficiency parameters of RAPD primers. Indian J. Biotechnol. 2010. Vol. 9. P. 276–282