Definitions of antibodies to the newcastle disease virus in the yolk of birds of artificial nesting box in conditions of the North-East of Ukraine

Main Article Content

E. O. Yarys
E. S. Kolesnik
D. V. Muzyka
A. B. Chaplygina


Introduction. In the conditions of the North-East of Ukraine, the circulation of the newcastle disease virus in hollow-nesting birds of artificial nests box was studied. Serological studies of the yolks of eggs collected from hollow birds in the North-East of Ukraine to the Newcastle disease virus were carried out.

Purposе. Definitions of antibodies to the newcastle disease virus in eggs of artificial nesting birds and determining their role as a reservoir of the causative agent of the disease in the North-East of Ukraine.

Methods. The work was carried out in 2019 from April to December in the department for the study of poultry diseases at the National Scientific Center "Institute of Experimental and Clinical Veterinary Medicine", as well as at the Department of Zoology at the H. S. Skovoroda Kharkiv National Pedagogical University. Serological studies of the yolks of eggs taken from a hollow nesting box of birds. Antibodies in egg yolk extracts from birds of artificial box nests against the virus of newcastle disease, were determined in the reaction of delayed hemagglutination (HAGA).

Result. Antibodies to the newcastle disease virus in titers 1:2 (3,3%) were detected in Phoenicurus phoenicurus, 1:16 (3,3%) ‒ Ficedula albicollis, 1:32 (10,0%) ‒ in the yolks of Erithacus rubecula eggs and Phoenicurus phoenicurus, titers 1:64 (23,3%) and 1:128 (26,6%) were found in the yolks of all studied species of artificial nesting birds. The highest antibody titers are 1:256 (26,6%) and 1:512 (6,6%) in the yolks of Ficedula albicollis and Phoenicurus phoenicurus eggs.

Originality. Discovered of antibodies to the newcastle disease virus in the yolk of birds of artificial nesting box in conditions of the North-East of Ukraine.

Conclusion. According to the results of serological studies of the eggs of birds of four species (Ficedula albicollis, Parus major, Erithacus rubecula and Phoenicurus phoenicurus), which nest in northeastern Ukraine, it was found that antibodies to the Newcastle disease virus were found in 30 (66.6%).

Article Details



Vorotilova, N. G., & Ionkina, I. B. (2013). Study of the role of pigeons in the spread of Newcastle disease virus in the Crimea. Scientific works of the Southern branch of the National University of Life and Environmental Sciences of Ukraine "Crimean Agrotechnological University". Seriia: Veterynarni nauky. [Series: Veterinary sciences]. 155, 63-69 (in Ukr).

Glushchenko, A. V., Yurchenko, K. S., Yurlov, A. K., Shchelkanov, M. Yu., & Shestopalov, A. M. (2016). On the role of wild birds in the conservation and spread of avian paramyxovirus serotype 1 (Newcastle disease virus) in Siberia and the Far East. Yuh Rossyy: эkolohyia, razvytye. [South of Russia: Ecology, Development]. 11(2), 50-58. doi: 10.18470/1992-1098-2016-2-50-58

Muzyka, D. V., Stegniy, B. T., & Bezrukava, I. Yu. (2002). Epizootological monitoring of wild waterfowl in the Askania-Nova Biosphere Reserve by the method of studying egg yolk extracts. Vet. medytsyna: Mizhvid. temat. nauk. zb. [Vet. medicine: Interdepartmental. topic. science. zb]. Kh. 80, 439-442 (in Ukr).

Muzyka, D. V., & Chaplygina, A. B. (2015). The results of immunological studies of some background birds of North-Eastern Ukraine for the presence of antibodies to ortho- and paramyxoviruses. Sb. nauchn. trudov Azovo-Chernomorskoi ornytolohycheskoi stantsyy «Branta». [Sat. scientific works of the Azov-Black Sea ornithological station "Branta"]. 18, 133-140 (in Ukr).

Nedzinskas, V. (1972). Egg-laying and determination of the degree of hatching of whooper swan eggs. Thesis. report 8th Baltic, ornithol. conf. Tallinn,76-77 (in Rus).

Fotin, A. I., Panasenko, O. S., & Panasenko, O. A. (2010). Analysis of potential dangers of ostrich viral infections in Ukraine. Bulletin of Sumy National Agrarian University. Seriia «Veterynarna medytsyna». [Series «Veterinary Medicine»]. Sumy, 3(26), 126-131 (in Ukr).

Chu, H. P., Trow, E. W., Greenwood, A. G., Jennings, A., & Keymer, I. F. (1976). Isolation of Newcastle disease virus from birds of prey. Avian Pathology, 5, 227-233. doi:10.1080/03079458108418488

Dimov, I. D. (2011). Study on the degree of parasitism of rhinonyssid nasal mites (Parasitiformes: Gamasina) on birds in the Leningrad province during the spring and summer seasons. Trakia Journal of Sciences, 9(2), 3842 p. (in Rus).

Hoque, M. A., Burgessa, G. W., Karo-Karo, D., Cheam, A. L., & Skerratt, L. F. (2012). Monitoring of wild birds for Newcastle disease virus in north Queensland, Australia. Preventive Veterinary Medicine, 103, 49-62. doi: 10.1016/j.prevetmed.2011.08.013

Keymer, I. F. (1975). Classification of birds and host list of important diseases. In: Bird Diseases. An introduction to clinical diagnosis and treatment of diseases in birds other than poultry. Arnall, L. and Keymer, I.F. T.F.H. Publications, Inc., Ltd. Published in Great Britain by Baillière Tindall, London, 451-460 (UK).

Sharma, B., Pokhriyal, M., Gaurava, K. R., Saxena, M., Ratta B., …Mondal, B. (2012). Isolation of Newcastle disease virus from a non-avian host (sheep) and its implications. Archives of Virology, 157, 1565-1567. doi: 10.1007/s00705-012-1317-8

Shchelkanov, M. Y., Anan'ev, V. Y., L'vov, D. N., Kireev, D. E., Gur'ev, E. L., Akanina, D. S., …

Lvov D. K. (2007). Complex environmental and virological monitoring in the Primorje Territory in 2003 ‒ 2006. Voprosy virusologii, 52(5), 37-48 (in Rus).

Xiang, B., Han, L., Gao, P., You, R., Wang, F., Xiao, J., …Ren, T. (2017). Spillover of Newcastle disease viruses from poultry to wild birds in Guangdong province, southern China. Infect Genet Evol,55, 199-204. doi: 10.1016/j.meegid.2017.09.020

Yaqing, L., Chengxi, S., Miaomiao, C., Hongling, W., Li Z., Yanyan, S., Na L., & Zhiyu, W. (2019). Genetic characterization and phylogenetic analysis of Newcastle disease virus from China. Infection, Genetics and Evolution, 75, 103958 p. doi: 10.1099/jgv.0.001349

Zhao, P., Sun, L., Sun, X., Li, S., Zhang, W., Pulscher, L., Chai, H., & Xing, M. (2017). Newcastle disease virus from domestic mink, China, 2014. Veterinary Microbiology, 198, 104-107. doi: 10.1016/j.vetmic.2016.12.003