Effect of l-type calcium channel blockade on respiratory pattern and gas exchange in rats on induced insulin resistance
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Abstract
in experiments on 24 male rats, the effect of blockade of slow calcium channels on respiration and gas exchange was studied in modeling insulin resistance. Insulin resistance was simulated using a high-fat diet (HFD) for 2 weeks. Slow calcium channels were blocked by verapamil, which was administered intraperitoneally in a generally accepted dose of 1 mg/kg of the animal's body weight. Research on breathing patterns and gas exchange was carried out using a device that included a breathing mask, a pneumotachograph for small laboratory animals and a mass spectrometer. Respiratory rate (f) and respiratory volume (VT) were measured, minute respiratory volume (VE) was calculated. Using a mass spectrometer, the amount of consumed oxygen (VO2) and released carbon dioxide (VCO2) per minute was determined.
It is shown that with the introduction of a blocker of slow calcium channels, an increase in the tone of the bronchioles and stenoventilatory restructuring of breathing is observed, without changes in probable changes in energy metabolism. It was found that the use of verapamil in rats with simulated insulin resistance causes an increase in bronchial tone, a deepening of the stenoventilatory remodeling of breathing and a decrease in the delivery of oxygen to the alveoli of the lungs, which is accompanied by a decrease in the level of energy metabolism.
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