The expression of endothelial NO-synthase (eNOS) in monocytes and platelets during the adaptation to physical loads

Article Sidebar

PDF (Українська)
Published: січ. 23, 2016
Keywords:
gene expression, endothelial NO-syntase, adaptation to physical exersices, T -786 → C polymorphisme NOS gene

Main Article Content

S. B. Drozdovska
Національний університет фізичного виховання і спорту України

Abstract

To establish the physiological role of thee NOS gene in adaptation to muscular work and effect mechanism of genepolymorphism, blood cellse NOS gene expression in adapted to different types of work athletes and the physical change under in fluence of physical activity were studied. Level of eNOS expression was determined in monocytes and blood platelets 33 athletes and 10 sedentary men, not adapting to systematic physical activity. DNA was isolated from buccal epithelium. RNA was extracted from platelets and monocytes. T -786 → C polymorphis mand eNOS gene expression were detected by Real Time PCR. For an assay of eNOS enzyme activity in platelets we used a fluorimetric detection system FCANOS-1 (Sigma). Higher level of eNOS gene expression and eNOSenzyme activity are observed in athletes’ platelets compared to people uninvolved in sports (20,8% fold and 22,8%, p<0,01 respectively). We found that the levels eNOS mRNA of blood cells in athletes, adapted to the aerobic exercises in the rest was higher, than athletes adapted to the anaerobic exercises. eNOS mRNA of blood cells and NO – synthase activity in platelets were lower than in monocytes, as in the control group and athletes. Physic alactivity leads to the increase of the eNOS gene expression and NO – synthase activity in platelets and decrease in blood monocytes qualified athletes. Adaptation to physical exercise with various pathways of energy supply results in different in value changes of eNOS gene expression level in the rest. Therefore, eNOS affects the development of adaptation processes to physical exercise among qualified athletes. Apparently, high demand of NO during endurance exercises is related to its modifying effect on carbohydrates and oxygen consumption and its influence on basal mitochondrial biogenesis in skeletal muscles.

Article Details

Issue
Vol. 335 No. 2 (2015): Visnyk Cherkas'koho universytetu: seriya biolohichni nauky [Cherkasy University Bulletin: Biological Sciences series]
Section
Статті

Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.

AGREEMENT ABOUT TRANSMISSION OF COPYRIGHT

I, the author of the article / We, the authors of the manuscript _______________________________________________________________________

in case of its acceptance for publication, we transfer the following rights to the founders and editorial boards of the scientific publication "Cherkasy University Bulletin: Biological Sciences Series":

1. Publication of this article in Ukrainian (English) and distribution of its printed version.

2. Dissemination of the electronic version of the article through any electronic means (placing on the official journal web site, in electronic databases, repositories, etc.). At the same time we reserve the right without consent of the editorial board and the founders:

1. Use the materials of the article in whole or in part for educational purposes.

2. To use the materials of the article in whole or in part for writing your own theses.

3. Use article materials to prepare Summarys, conference reports, and oral presentations.

4. Post electronic copies of the article (including the final electronic version downloaded from the journal's official website) to:

a. personal web-pecypcax of all authors (web sites, web pages, blogs, etc.);

b. web-pecypcax of the institutions where the authors work (including electronic institutional repositories);

with. non-profit, open-source web-pecypcax (such as arXiv.org).

With this agreement, we also certify that the submitted manuscript meets the following criteria:

1. Does not contain calls for violence, incitement of racial or ethnic enmity, which are disturbing, threatening, shameful, libelous, cruel, indecent, vulgar, etc.

2. Does not infringe the copyrights and intellectual property rights of others or organizations; contains all the references to the cited authors and / or publications envisaged by applicable copyright law, as well as the results and facts used in the article by other authors or organizations.

3. It has not been previously published in other publishers and has not been published in other publications.

4. Does not include materials that are not subject to publication in the open press, in accordance with applicable law.

____________________ ___________________

First name, Last name,  signature of the author

"___" __________ 20__

References

Богдановська Н.В. Оксид азоту як регулятор адаптивних можливостей організму практично здорових юнаків і дівчат/ Н.В. Богдановська// Вчені записки Таврій. нац. ун-ту ім. В. І. Вернадського. – 2012. – Т.25, №4. – С.3-11.

Богдановська Н.В. Синтез оксиду азоту у період довгострокової адаптації до інтенсивної м’язової роботи у спортсменок / Н.В. Богдановська, Г.М. Святодух, А.В. Коцюруба та ін. // Фізіол. журн. − 2009. − 55(3). − С. 94-99.

Марышева Е.Ф. Тромбоцитарный гемостаз при физической нагрузке:автореф.дис. …канд.биол.наук: спец. 03.00.13»/ Е.Ф. Марышева. Тюмень. 2003. 26 с.

Фаллер Д.М. Молекулярная біологія клетки: рук. для врачей / Д.М. Фаллер, Д. Шилдс.– М.: БИНОМ, 2011. –256 с.

AiLunY.Chronic Exercise Increases Both Inducible and Endothelial Nitric Oxide Synthase Gene Expressionin Endothelial Cells of Rat Aorta /Y. Ai-Lun, T.Shaw-Jenq, J.MeeiJyhet al.//J. of Biomedical Science. – 2002. – Vol. 9,N. – Р.149.

Bailey S.J. The nitrate-nitrite-nitric oxide pathway: Its role in human exercise physiology / S.J. Bailey, A.Vanhatalo, P.G. Winyard et al. // European Journal of Sport Science. – 2012. – Vol. 12,N 4. – P.309-320.

Bescós R. The Effect of Nitric-Oxide-Related Supplements on Human Performance/ R.BescósA.Sureda; J. A.Tur; A.Pons// Sports Medicine. – 2012. – Vol.42, Is. 2. –Р. 99-117.

Bradley S. J. Nitric oxide synthase inhibition reduces leg glucose uptake but not blood flow during dynamic exercise in humans / S. J. Bradley, B.A. Kingwell, G.K. McConell // Diabetes. – 1999. – №48. – Р. 1815-21.

Cortese-Krott M.M. Human red blood cells at work: identification and visualization of erythrocytic activity in health and disease/ M.M. Cortese-Krott, A.Rodriguez-Mateos, R.Sansone et al. // Blood. – 2012. – № 120. – P.4229-4237.

Cruz-González I. Association between -T786C NOS3 polymorphism and resistant hypertension: a prospective cohort study /I. Cruz-González, E. Corral, M. Sánchez-Ledesma et al. // BMC Cardiovascular Disorders. – 2009. – № 9. – Р.35.

Erbs S. Promoter but not exon polymorphism of endothelial nitric oxide synthase affects training-induced correction of endothelial dysfunction / S. Erbs , Y.Baither , A. Linke et al. // Arterioscler. Thromb. Vasc. Biol. – 2003. – Vol.23. – P.1814-1819.

FukaiT.Regulation of the vascular extracellular superoxide dismutase by nitric oxide and exercise training/ T.Fukai, M.R. Siegfried, M.Ushio-Fukai et al. // J.Clin.Invest. –2000. –N. 105. –P.1631-1639.

Green D.J. Effect of exercise training on endothelium-derived nitric oxide function in humans/ D.J. Green, A. Maiorana, G.O'Driscoll et al.// J.Physiol. –2004. –N. 561. – P.1-25.

Grijalva J. Exercise training enhanced myocardial endothelial nitric oxide synthase (eNOS) function in diabetic Goto-Kakizaki (GK) rats/ J. Grijalva, S. Hicks, X. Zhao et al. //Cardiovascular Diabetology. –2008. – N 7. – P.7-34.

Hussain S.N. Expression of nitric oxide synthase isoforms in normal ventilatory and limb muscles/ S.N. Hussain, Q.El-Dwairi, M.N. Abdul-Hussain // J. Appl.Physiol. –1997.–Vol.83. – P. 348-53.

Jayachandran M.Ovariectomy upregulates expression of estrogen receptors, NOS, and HSPs in porcine platelets / M.Jayachandran, V.M. Miller // Amer. J. Physiol. Heart Circ. Physiol. – 2002. –Vol.283. – P.220–226.

Kojda G. Dysfunctional regulation of Endothelial Nitric Oxide Synthase (eNOS) expression in response to exercise in mice lacking one eNOS gene/ G.Kojda, Y.C. Cheng, J. Burchfield et al. // Circulation. –2001. – N 103. – P.2839-2844.

Lau KS.nNOS and eNOS modulate cGMP formation and vascular response in contracting fast-twitch skeletal muscle/ Lau KS, Grange RW, Isotani E et al.// Physiol.Genomics. – 2000. –V. 2.– P. 21-7.

Le Cras T.D. Effects of chronic hypoxia and altered hemodynamics on endothelial nitric oxide synthase expression in the adult rat lung/ T.D. Le Cras, R.C. Tyler, M.P.Horan et al.// J. Clin. Invest. – 1998. –N 101. – Р. 795–801.

McConell G.K. Potential role nitric oxide in contraction-stimulated glucose uptake and mitochondrial biogenesis in skeletal muscle / G.K. McConell, G.D.Wadley // Proceeding of the Australian Physiological Society. – 2008. – N 39. – P.69-74.

Niess A.M. Physical exercise-induced expression of inducible nitric oxide synthase and heme oxygenase-1 in human leukocytes: effects of RRR-alpha-tocopherol supplementation /A.M. Niess, M. Sommer, M. Schneider et al. //Antioxid Redox Signal. – 2000. – 2, N1. – P. 113-26.

Saluja R. Molecular and biochemical characterization of nitric oxide synthase isoforms and their intracellular distribution in human peripheral blood mononuclear cells/ R.Saluja, A. Jyoti, M.Chatterje et al. // Biochimica et biophysicaActa. – 2011. – N.1813. – P.1700-1707.

Shantsila R. The effects of exercise and diurnal variation on monocyte subsets and monocyte-platelet aggregates/ R. Shantsila Eduard, Luke D. Tapp, Benjamin J. Wrigley, Silvia Montoro-Garcia, Angie Ghattas, Anthony Jaipersad and Gregory Y. H. Lip //European Journal of Clinical Investigation. 2012. Р. 1-8.

Suvorava T. Physical inactivity causes endothelial dysfunction in healthy young mice/ T. Suvorava, N.Lauer, G.Kojda// J.Am.CollCardiol. – 2004. – N 44. – P.1320-7.

Tanabe T. Exercise training improves ageing-induced decrease in eNOS expression of the aorta /T. Tanabe , S. Maeda, T. Miyauchi et al. //ActaPhysiol Scand.– 2003.– 178 (1).– p.3-10.

Toporsian M. Downregulation of endothelial nitric oxide synthase in rat aorta after prolonged hypoxia in vivo/M.Toporsian, K.Govindaraju, M.Nagi et al. //Circ Res.–2000.– 86. – Р. 671–675.

Wadley G.D. NOS isoformspecific regulation of basal but not exercise-induced mitochondrial biogenesis in mouse skeletal muscle/ G.D. Wadley, J.Choate, G.K. McConell // J. Physiol.– 2007. –Vol. 585. – P. 253-62

Walther C. The Effect of Exercise Training on Endothelial Function in Cardiovascular Disease in Humans/Claudia Walther, Stephan Gielen, Rainer Hambrecht //Exerc Sport Sci Rev. – 2004. – Vol. 32, N4. – P.130-134.

Wilkerson D.P. Influence of nitric oxide synthase inhibition on pulmonary O2 uptake kinetics during supra-maximal exercise in humans/ D.P. Wilkerson, I.T. Campbell, A.M. Jones // J. Physiol.– 2004. – Vol. 561, N 2. – P.623-635.

Wolfarth B Endothelial nitric oxide synthase gene polymorphism and elite endurance athlete status: the Genathlete study/ B.Wolfarth, T.Rankinen, S.Mühlbauer et al. // Scand J Med Sci Sports. – 2008.– N 18. –485–490.