Features of the qualitative composition of bile of dogs under the action of different doses of glycine

Article Sidebar

PDF (Українська)
Published: січ. 25, 2016
Keywords:
glycine, bile secretion, taurocholate, conjugated bile acid, free bile acids, cholesterol, chronic experiment

Main Article Content

Z. A. Gorenko
НДІ фізіології імені академіка Петра Богача ННЦ «Інститут біології» Київського національного університету імені Тараса Шевченка

Abstract

In chronic experiments on mongrel dogs with implanted combined cholecysto-duodenal fistula tubes studied the effect of glycine on the bile flow rate and qualitative composition of bile. Within three hours of observation, the intensity and concentration of bile secretion in its free and conjugated bile acids, and free cholesterol by thin layer chromatography. It is shown that for an 8-day oral administration at doses of 4.9 or 9.7 mg/kg body weight of the amino acid shows no choleretic impact, thus changing the quality of the secreted bile. It has been established that a dose of 4.9 mg/kg of glycine conjugation enhances trihydroxy- and digidroksiholates with taurine, and does not affect secretion of free cholesterol and cholates. This is accompanied by an increase in the values of cholate/cholesterol ratio, which indicates an improvement solubilization properties of bile and decrease its lithogenicity. At a dose of 9.7 mg/kg of this amino acid intensifies the secretion of the bile conjugated and unconjugated cholates, as well as free cholesterol, which generally contributes to the conservation colloidal properties of bile and prevents nucleation of cholesterol in the aqueous phase secretion, ie the formation of stones in the biliary ways.

Article Details

Issue
Vol. 295 No. 2 (2014): Visnyk Cherkas'koho universytetu: seriya biolohichni nauky [Cherkasy University Bulletin: Biological Sciences Series]
Section
Статті

Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.

AGREEMENT ABOUT TRANSMISSION OF COPYRIGHT

I, the author of the article / We, the authors of the manuscript _______________________________________________________________________

in case of its acceptance for publication, we transfer the following rights to the founders and editorial boards of the scientific publication "Cherkasy University Bulletin: Biological Sciences Series":

1. Publication of this article in Ukrainian (English) and distribution of its printed version.

2. Dissemination of the electronic version of the article through any electronic means (placing on the official journal web site, in electronic databases, repositories, etc.). At the same time we reserve the right without consent of the editorial board and the founders:

1. Use the materials of the article in whole or in part for educational purposes.

2. To use the materials of the article in whole or in part for writing your own theses.

3. Use article materials to prepare Summarys, conference reports, and oral presentations.

4. Post electronic copies of the article (including the final electronic version downloaded from the journal's official website) to:

a. personal web-pecypcax of all authors (web sites, web pages, blogs, etc.);

b. web-pecypcax of the institutions where the authors work (including electronic institutional repositories);

with. non-profit, open-source web-pecypcax (such as arXiv.org).

With this agreement, we also certify that the submitted manuscript meets the following criteria:

1. Does not contain calls for violence, incitement of racial or ethnic enmity, which are disturbing, threatening, shameful, libelous, cruel, indecent, vulgar, etc.

2. Does not infringe the copyrights and intellectual property rights of others or organizations; contains all the references to the cited authors and / or publications envisaged by applicable copyright law, as well as the results and facts used in the article by other authors or organizations.

3. It has not been previously published in other publishers and has not been published in other publications.

4. Does not include materials that are not subject to publication in the open press, in accordance with applicable law.

____________________ ___________________

First name, Last name,  signature of the author

"___" __________ 20__

References

Амахин Д.В. Взаимодействие эффектов нейромедиаторов глицина и ГАМК в центральной нервной системе (Gaba and glycine — interaction of responces and receptor cross talk) / Д.В.Амахин, Н.П.Веселкин // Цитология. – 2012. – Т.54, № 6. – С.469-477.

А.с. 1624322 СССР, МБИ G 01 N 33/50 Способ определения желчных кислот в биологической жидкости / С.П.Весельский, П.С.Лященко, И.А.Лукьяненко (СССР); - № 4411066/14; заявл. 25.01.1988; опубл.30.01.1991, Бюл.№ 4.

Клюева Н.Н. Гиполипидемическая активность триметилглицина (Hypolipidemic activity of trimethylglycine) / Клюева Н.Н., Калашникова Н.М., Окуневич И.В., Сапронов Н.С. // Эксп. клин. фармакол. – 2012. – Т.75(1). – С.16-18.

Лященко П.С. К методике исследования внешнесекреторной функции печени у собак (To study the method of exocrine function of the liver in dogs) / Лященко П.С. // Физиол. журн. СССР. – 1975. - Т.60, № 12. – С.1891-1893.

Патент 33564 А Україна, МПК А 61В 5/14. Спосіб підготовки проб біорідин для визначення вмісту речовин ліпідної природи / Весельський С.П., Лященко П.С., Костенко С.І., Горенко З.А., Куровська Л.Ф.; заявник та власник патенту Київський університет імені Тараса Шевченка - № 99031324; заявл.11.03.99; опубл.15.02.2001, Бюл.№ 1.

Alcaraz-Conteras Y. Effect of glycine on lead mobilization, lead-induced oxidative stress, and hepatic toxicity in rats / Alcaraz-Conteras Y., Garza-Ocañas L., Carcaño-Diaz K., Ramirez-Gomez X.S. // J. Toxicol. – 2011. – V.2011. – ID430539. – 7 p.

Benk T. Glycine pretreatment ameliorates liver injury after partial hepatectomy in the rat / Benk T., Frede S., Gu Y. [et al.] // J. Invest. Surg. – 2010. – V.23, Is.1. – P.12-20.

Bhattacharyya S. Iron induces hepatocytes death via MAPK activation and mitochondria-dependent apoptotic pathway: beneficial role of glycine / Bhattacharyya S., Ghosh J., Sil P.C. // Free Radical Research. – 2012. – V.46 (10). – P.1296-1307.

Cassel M.D. Glycine receptor (gephyrin) immunoreactivity is present on cholinergic neurons in the dorsal vagal complex / Cassel M.D., Yi H., Talman W.T. // Neuroscience. – 2000. – V.95. – P.489-497.

El Hafidi M. Glycine intake decreases plasma free fatty acids, adipose cell size, and blood pressure in sucrose-fed rats / El Hafidi M., Perez I., Zamora J. [et al.] // Am. J. Physiol. Regul. Integr. Comp. Physiol. - 2004. – V.287. – P.R1387-R1393.

Fain J.N. Adrenergic regulation in adipose metabolism / Fain J.N., Garcia-Sainz J.A. // J. Lipid. Res. – 1983. – V.24. – P.945-966.

Ito K. Effect of non-essential amino acid glycine administration on the liver regeneration of partially hepatectomized rats with hepatic ischemia/reperfusion injury / Ito K., Ozasa H., Noda Y. [et al.] // Clin. Nutr. – 2008. – V.27, Is.5. – P.773-780.

Kawai N. Pharmacokinetics and cerebral distribution of glycine administered to rats / Kawai N., Bannai M., Seki S., [et al.] // Amino Acids. – 2012. – V.42(6). – P.2129-37.

Jobgen W.S. Regulatory role for the arginine-nitric oxide pathway in metabolism of energy substrates / Jobgen W.S., Fried S.K., Fu W.J. [et al.] // J. Nutr. Biochem. – 2006. – V.17. – P.571-588.

Lee M.A. Influence of glycine on intestinal ischemia-reperfusion injury / Lee M.A., McCauley R.D., Kong S.E., Hall J.C. // J.Parenter. Enteral. Nutr. – 2002. – V.26. – P.130-135.

Li C. Regulation of glukagon secretion in normal and diabetic human islets by γ-hydroxybutirate and glycine / Li C., Chen J., Chen P. [et al.] // J. Biol. Chem. – 2013. – Vol.288 (6). – P.393-351.

Li P. Amino acids and immune function / Li P., Yin Y.L., Li D.F. [et al.] // Br. J. Nutr. – 2007. – V.98. – P.237-252.

Malosio M.L. Widespread expression of glycine receptor subunit mRNAs in the adult and developing rat brain / Malosio M.L., Marqueze-Pouey B., Kuhse J., Betz H. // EMBO J. – 1991. – V.10. – P.2401-2409.

Martin P.M. Amino acids transport regulates blastocyst implantation / Martin P.M., Sutherland A.E., VanWinkle L.J. // Biol. Reprod. – 2003. – V.69. – P.1101-1108.

Meijer A.J. Amino acids as regulators and components of nonproteinogenic pathways / Meijer A.J. // J. Nutr. – 2003. – V.133. – P.2057S-2062S.

Mikalauskas S. Dietary glycine protects from chemotherapy-induced hepatotoxicity / Mikalauskas S., Mikalauskiene L., Bruns H. [et al.] // Amono Acids. – 2011. – V.40, Is.3. – P.1139-1150.

Newsholme P. New insights into amino acid metabolism, beta-cell function and diabetes / Newsholme P., Brennan L., Rubi B., Maechler P. // Clin. Sci. – 2005. – V.108. – P.185-194.

Oertel J. A novel glycine receptor beta subunit splice variant an unorthodox transmembrane topology. Assembly into heteromeric receptor complexes / Oertel J., Villmann C., Kettenmann H., [et al.] // J. Biol. Chem. – 2007. – V.282. – P.2798-2807.

Pal P.B. Modulation of mercury-induced mitochondria-dependent apoptosis by glycine in hepatocytes / Pal P.B., Pal S., Das J., Sil P.C. // Amino Acids. – 2012. – Vol.42(5). – P.1669-1683.

Schemmer P. Glycine reduces platelet aggregation / Schemmer P., Zhong Z., Galli U. [et al.] // Amino Acids. – 2013. – Vol.44(3). – P.925-931.

Schneider L. Effects of gadolinium chloride and glycine on hepatic and pancreatic tissue damage in alcoholic pancreatitis / Schneider L., Hackert T., Longerich T. [et al.] // Pancreas. – 2010. – V.39, Is.4. – P.502-509.

Tsune I. Dietary glycine prevents chemical-induced experimental colitis in the rat / Tsune I., Ikejima K., Hirose M. [et al.] // Gastroenterology. – 2003. – V.125, Is.3. – P.775-785.

Van den Eynden J. Glycine and glycine receptor signaling in non-neuronal cells / Van den Eynden J., Sheen Saheb Ali, Horwood N. [et al.] // Fronf. Mol. Neurosci. – 2009. - V.2. – P.9.

Wu G. Glutathione metabolism and its implication for health / Wu G., Fang Y.Z., Yang S. [et al.] // J. Nutr. – 2004. – V.1345. – P.489-492.

Yamanouchi K. Glycine reduces hepatic warm ischemia-reperfusion injury by suppressing inflammatory reactions in rats / Yamanouchi K., Eguchi S., Kamohara Y. [et al.] // Liver Int. – 2007. – V.27. – P.1249-1254.

Yue J.T.Y. Glycine normalizes hepatic triglyceride-rich VLDL secretion by triggering the CNS in high-fad rats / Yue J.T.Y., Mighiu P.I., Naples M. [et al.] // Circ.Res. – 2012. – V.110. – P.1345-1354.

Zhang S.J. Protective effect of glycine pretreatment on brain-death donor liver / Zhang S.J., Shi J.H., Tang Z. [et al.] // Hepatobiliary Pancreat. Dis. Int. – 2005. – V.4, Is.1. – P.37-40.