Features of the qualitative composition of bile of dogs under the action of different doses of glycine

Z. A. Gorenko

Abstract


In chronic experiments on mongrel dogs with implanted combined cholecysto-duodenal fistula tubes studied the effect of glycine on the bile flow rate and qualitative composition of bile. Within three hours of observation, the intensity and concentration of bile secretion in its free and conjugated bile acids, and free cholesterol by thin layer chromatography. It is shown that for an 8-day oral administration at doses of 4.9 or 9.7 mg/kg body weight of the amino acid shows no choleretic impact, thus changing the quality of the secreted bile. It has been established that a dose of 4.9 mg/kg of glycine conjugation enhances trihydroxy- and digidroksiholates with taurine, and does not affect secretion of free cholesterol and cholates. This is accompanied by an increase in the values of cholate/cholesterol ratio, which indicates an improvement solubilization properties of bile and decrease its lithogenicity. At a dose of 9.7 mg/kg of this amino acid intensifies the secretion of the bile conjugated and unconjugated cholates, as well as free cholesterol, which generally contributes to the conservation colloidal properties of bile and prevents nucleation of cholesterol in the aqueous phase secretion, ie the formation of stones in the biliary ways.

Keywords


glycine; bile secretion; taurocholate; conjugated bile acid; free bile acids; cholesterol; chronic experiment

References


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