Influence of medicine “Cordyceps & Lingzhi” and its fractions on some indexes of immune system, tumor growth and survival of mice with Ehrlich’s carcinoma

S. P. Veselsky, R. S. Dovgiy, V. N. Svyatetska, M. P. Rudyk, V. V. Pozur, A. N. Makarenko

Abstract


Fungotherapy is a well-developed method of therapy in East Asia. Nowadays there is the search and study of the main active substances with different pharmacological activities from higher fungi takes place.  The aim of our study was to compare the immunomodulatory and antitumor activity of the medicine "Cordyceps and Lingzhi" and water-and fat-soluble fractions derived from it. In vitro studies conducted using cells obtained  from outbred mice. In vivo studies conducted on Balb/c mice. Functional activity of phagocytes was determined using the nitroblue tetrazolium test. The reaction of lymphoid organs was measured by indexes of their relative weight and relative cellularity. The presence of immunomodulating activity of “Cordyceps & Lingzhi” medicine water and fat soluble fractions was shown. Changes of oxygen-dependent metabolism were the most reliable, they were increased at addition of analyzed fractions in all concentrations. Nevertheless, application of non-fractionated medicine “Cordyceps & Lingzhi” had more marked effects in almost all investigated parameters, such as oxygen-dependent metabolism (19-fold increase at addition of 100 mkg/ml of medicine, as compared to control), indexes of lymphoid organs (1,5-fold increase of relative weight of lymph nodes), dynamics of tumor growth (statistically significant inhibition of tumor growth by 24% as compared to control).


Keywords


“Cordyceps & Lingzhi”; water soluble fraction; fat soluble fraction; immune system; tumor

References


Patel S. Recent developments in mushrooms as anti-cancer therapeutics: a review / S. Patel, A. Goyal // Biotech. – 2012. – V. 2, №1. – P. 1–15.

Zhou X. Cordyceps fungi: natural products, pharmacological functions and developmental products / X. Zhou, Z. Gong, Y. Su [et al.] // Journal of Pharmacy and Pharmacology. – 2009. – V. 61. – P. 279 – 291.

Rao Y.-K. Constituents isolated from Cordyceps militaris suppress enhanced inflammatory mediator’s production and human cancer cell proliferation / Y.-K. Rao, S.-H. Fang, W.-S. Wu, Y.-M. Tzeng – .J Ethnopharmacol. – 2010. – V. 131. – P. 363–367.

Chen J. Medicinal importance of fungal β-(1 → 3) (1 → 6)-glucans / J. Chen, R. Seviour // Mycol. Res. – 2007. – V. 111. – P. 635–652

Wang P-Y. Antitumor and Immunomodulatory Effects of Polysaccharides from Broken-Spore of Ganoderma lucidum / P.-Y. Wang, X.-L. Zhu, Z.-B. Lin // Front Pharmacol. – 2012. – V. 3. – P. 135.

Lin S.B. Triterpene-enriched extracts from Ganoderma lucidum inhibit growth of hepatoma cells via suppressing protein kinase C, activating mitogen-activated protein kinases and G2-phase cell cycle arrest / S.B. Lin, C.H. Li, S.S. Lee, L.S. Kan // Life Sci. – 2003. – V. 72. – P. 2381 – 2390.

Галиев Х.Г. Микрометод определения ароматических аминокислот в субклеточных структурах печени / Х.Г. Галиев // Экспериментальная патология печени. – 1981. – №4. – С. 245 – 250.

Пат. 99031324 Україна, А 61В 5/14 Спосіб підготовки проб біорідин для визначення вмісту речовин ліпідної природи / С.П. Весельський; Київський Університет імені Тараса Шевченка. 15.02.2001. Бюл. №1.

Резников О. Проблеми етики при проведенні експериментальних медичних і біологічних досліджень на тваринах / О. Резников // Вісн. НАН України. – 2001. - №1. – С. 5-7.

Sensitivity of mouse lymphoid and nonlymphoid organs to Silesian air pollutants / E. Kozlowska, J. Kopec-Szlezak, N. Drela// Ecotoxicol. Environ. Saf. - 1997. - №37. - №1. - P.10-16.

Реброва О.Ю. Статистический анализ медицинских данных / О.Ю. Реброва. – Москва: МедиаСфера, 2002. – 312 с.


Full Text: PDF (Українська)

Refbacks

  • There are currently no refbacks.
Archive
2014 2 36
2015 2 19
2016 1 2
2017 1 2
2018 1 2
2019 1  

User

Information

Journal Content

Browse

Language