The expression of endothelial NO-synthase (eNOS) in monocytes and platelets during the adaptation to physical loads

S. B. Drozdovska

Abstract


To establish the physiological role of thee NOS gene in adaptation to muscular work and effect mechanism of genepolymorphism, blood cellse NOS gene expression in adapted to different types of work athletes and the physical change under in fluence of physical activity were studied. Level of eNOS expression was determined in monocytes and blood platelets 33 athletes and 10 sedentary men, not adapting to systematic physical activity. DNA was isolated from buccal epithelium. RNA was extracted from platelets and monocytes. T -786 → C polymorphis mand eNOS gene expression were detected by Real Time PCR. For an assay of eNOS enzyme activity in platelets we used a fluorimetric detection system FCANOS-1 (Sigma). Higher level of eNOS gene expression and eNOSenzyme activity are observed in athletes’ platelets compared to people uninvolved in sports (20,8% fold and 22,8%, p<0,01 respectively). We found that the levels eNOS mRNA of blood cells in athletes, adapted to the aerobic exercises in the rest was higher, than athletes adapted to the anaerobic exercises. eNOS mRNA of blood cells and NO – synthase activity in platelets were lower than in monocytes, as in the control group and athletes. Physic alactivity leads to the increase of the eNOS gene expression and NO – synthase activity in platelets and decrease in blood monocytes qualified athletes. Adaptation to physical exercise with various pathways of energy supply results in different in value changes of eNOS gene expression level in the rest. Therefore, eNOS affects the development of adaptation processes to physical exercise among qualified athletes. Apparently, high demand of NO during endurance exercises is related to its modifying effect on carbohydrates and oxygen consumption and its influence on basal mitochondrial biogenesis in skeletal muscles.

Keywords


gene expression; endothelial NO-syntase; adaptation to physical exersices; T -786 → C polymorphisme NOS gene

References


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