Lipid ratio in the bile of male rats under the influence of estrone
Introduction. Bile formation is a complex physiological and biochemical process. Bile formation regulated by many physiological factors. Hormones play an important role in regulating the formation and secretion of bile. The secretion of bile is carried liver cells – hepatocytes and cholangiocytes. These cells are sensitive to regulatory impact of sex hormones. It is known that estrogens affect on the composition of bile. We also know that women often suffer from cholelithiasis and cholestatic liver injury. It should be noted that estradiol regulates lipid metabolism in the whole body and liver cells in particular. So we investigate the role of estrogen in the regulation of metabolic transformation and transport of lipids in the liver cells. Nowadays the least studied effects are estrone effects on the liver. In addition, the role of estrogen in the regulation of functions hepato-biliary system in males are still not well studied.
Purpose. The purpose of our study was to investigate the effect of estrone on the secretion of bile lipids in male rats.
Methods. In the article the analysis of estron influence on the bile lipids secretion in the rats males is carried. Influence of estron (8 mkg per 100 g of body weight, intraportal, once in the acute experiment) on secretion of the bile lipids was studied in the acute experiments in male rats. The concentrations of the five fractions of lipids in the rat bile (phospholipids, cholesterol, cholesterol esters, free fat acids and triglycerides) were determine in the six samples of the bile using thin layer chromatography.
Results. Еstrone in male rats reduces concentration of phospholipids (by 23,0 – 24,7% relative to control) and cholesterol (by 13,5 – 24,2% relative to control) in the bile of male rats. But estrone increases the concentration of free fatty acids (by 45,2 – 96,7% relative to control) and esters of cholesterol (by 52,8 – 70,7% relative to control) in bile of male rats. Estron didn’t influenced on the concentration of the triglycerides in rats bile. Lipid fractions significantly changed under the influence of estrone. Holato-cholesterol ratio bile male rats, which affected estrone, more than animals in the control group.
Originality. It was revealed that estron decrease the concentration of phospholipids and cholesterol in the bile of male rats. But the concentration of free fatty acids and esters of cholesterol in bile male rats significantly exceeds the control values under the influence of estron. Also we found that holato-cholesterol coefficient of male rats bile under the influence of estron increased compared with the control. Thus, it was shown that estron modifies physiological and biochemical processes of lipid metabolism in the liver of male rats and affects the secretion of bile lipids.
Conclusion. Thus, estron influence on hepatic metabolism, transport and secretion of the lipids into the male rats bile. Estron does not increase lithogenic characteristics of the male rats bile.
Vovk, Ye.I. Gallstones in the XXI century: the treatment or prevention? (2010) Gastroenterologiya (Gastroenterology). 2, 3-10 (in Ukr.)
Shcherbynina, M.B. Gallstone disease in Ukraine: discussion and unresolved issues (2010) Zdorov`ya Ukrayiny (Health of Ukraine), 12-13 (in Ukr.)
Brady, C.W. Liver disease in menopause (2015) World J. Gastroenterol., 21(25), 7613-7620
Guy, J. Liver Disease in Women: The Influence of Gender on Epidemiology, Natural History, and Patient Outcomes (2013) Gastroenterol Hepatol (N Y), 9(10), 633–639
Stinton, L.M., Shaffer, E.A. Epidemiology of Gallbladder Disease: Cholelithiasis and Cancer (2012) Gut Liver, 6(2), 172–187
Bravo, E., Cantafora, A., Avella, M., Botham, KM. Metabolism of сhylomicron сholesterol іs delayed by estrogen. An іn vivo study in the rat (2001) Exp. Biol. Med., 226, 112-118
Cirillo, D.J., Wallace, R.B., Rodabough, R.J. et al Effect of Estrogen Therapy on Gallbladder Disease (2005) 293 (3), 330-339
Kalala, S., Krishna, M., Shah, R., Lebovics, E., Frishman, W. H. Pоstmenopausal hormone replacement therapy and the risk of calculous gallbladder disease (2006) Clinical Geriatrics., 14 (3), 25-29
Jirsa, M., Bronský, J., Dvořáková, L. et al ABCB4 mutations underlie hormonal cholestasis but not pediatric idiopathic gallstones (2014) World J. Gastroenterol., 20 (19), 5867–5874
Sundaram, S.S. Sokol, R.J. The multiple facets of ABCB4 (MDR3) deficiency (2007) Curr. Treat. Options Gastroenterol., 10(6), 495–503
de Bari, O., Wang, T.Y., Liu, M., Portincasa, P., Wang, D.Q. Estrogen induces two distinct cholesterol crystallization pathways by activating ERα and GPR30 in female mice (2015) J. Lipid. Res., 56 (9), 1691-1700
de Bari, O., Wang, T.Y., Liu, M., Portincasa, P., Wang, D.Q. The deletion of the estrogen receptor α gene reduces susceptibility to estrogen-induced cholesterol cholelithiasis in female mice (2015) Biochim Biophys Acta. Res., 10 (Pt A), 2161-2169
Korochina. I.E. Gastrointestinal aspects of the metabolic syndrome (2008) Ros. Zhurnal gastroenterologii, gepatologii, koloproktologii (Russ. Journal of gastroenterology, hepatology, coloproctology), 18, 26-37 (in Russ.)
Borovec, О.V., Bondzik, O.V., Reshetnik, E.M., Veselsky, S.P., Makarchuk, M.Yu. Bile secretion function of rats with estrone action in terms of the blockade of estrogen receptor tamoxifen (2013) Visnyk Cherkaskogo universytetu. Seria Biologichni nauky (Bulletin of Cherkasy University. Series of Biological Sciences), 2 (255), 8-15 (in Ukr.)
Borovec. О.V., Bondzik, O.V., Reshetnik, E.M., Veselsky, S.P., Makarchuk, M.Yu. The action of estrone on the secretion of lipid components of bile in the nature of female rats (2014) Naukovyi Visnyk Shidnoevropeiskogo universytetu imeni Lesi Ukrainky. Seria Biologichni nauky (Lesya Ukrainka Eastern European National University. Series of Biological Sciences), 13 (290), 101-105 (in Ukr.)
Klymiuk, О.V., Bondzik, O.V., Reshetnik, E.M., Veselsky, S.P., Makarchuk, M.Yu. Features bile formation in rats of different sex under the influence of estrone (2011) Fiziologichnyi zhurnal (Physiological journal), 57 (6), С.52-58 (in Ukr.)
Veselskiy, S.P., Lyaschenko, P.S., Lukyanenko, I.A.. A method of determining bile acids in biological fluids: A.s.4411066/14 SSSR, MBI G 01 N 33/50. #1624322; Zayavleno 25.01.1988; Opubl. 30.01.1991, Byul. # 4 (in USSR).
Filimonova, N.B., Fil, I.O., Mykhailova, T.S. (2004). Statistical analysis of data pursuant to the science-based medicine. Initial analysis of quantitative data, experimental results presentation. Pervynnyi analiz kilkisnykh danykh, podannia rezultativ eksperymentu. Medytsyna zaliznychnoho transportu Ukrainy (Medicine Railway Transport of Ukraine), 4, 30-38 (in Ukr.)
Filimonova, N.B., Fil, I.O. (2005). Statistical analysis of data pursuant to the science-based medicine. Comparison groups in quantitative terms. Medytsyna transportu Ukrainy (Medical transport Ukraine), 4, 86-93 (in Ukr.)
Ganitkevich, J.V. & Karbach, J.I. (1985). Investigation of bile. Biochemical and biophysical methods. Vyshcha shkola (High school), 136. (in Russ.)
Gromashevska, L.L., Miroshnychenko, V.P., Sidenko, O.Yu. (2000). Determination of bile acids, cholesterol in bile. Holato-cholesterol ratio. Lab.diagnostyka (Lab. diagnostic), 4, 46-48 (in Ukr.)
Dikkers, A., Tietge, U.J.F. Biliary cholesterol secretion: More than a simple ABC (2010) World J. Gastroenterol., 16(47), 5936–5945
Schwabe, R.F., Maher, J.J. Lipids in liver disease: looking beyond steatosis (2012) Gastroenterology., 142(1), 8-11
Gooijert, K.E., Havinga, R., Wolters, H. et al. The mechanism of increased biliary lipid secretion in mice with genetic inactivation of bile salt export pump (2015) Am. J. Physiol. Gastrointest. Liver Physiol., 308(5), G450-457
- There are currently no refbacks.